pdhC [2019-05-09 17:50:43]

pyruvate dehydrogenase (dihydrolipoamide acetyltransferase E2 subunit)

Locus: BSU14600

Isoelectric point: 4.86

Molecular weight: 47.38 kDa

Protein length: 442 aa

Gene length: 1329 bp

Function: links glycolysis and TCA cycle

Product: pyruvate dehydrogenase (dihydrolipoamide acetyltransferase E2 subunit)

Essential: no

E.C.: 2.3.1.12

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → TCA cycle
• Information processing → Genetics → Newly identified competence genes
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,530,537 1,531,865

Phenotypes of a mutant

• defects in sporulation and unable to grow on glucose as single carbon source PubMed
• poor growth PubMed
• non-transformable PubMed

The protein

Catalyzed reaction/ biological activity

• Acetyl-CoA enzyme N(6)-(dihydrolipoyl)lysine = CoA enzyme N(6)-(S-acetyldihydrolipoyl)lysine (according to Swiss-Prot)

Protein family

• lipoyl-binding domain (according to Swiss-Prot)

Paralogous protein(s)

• AcoC, OdhB, BkdB

Kinetic information

• Michaelis-Menten PubMed

Modification

• phosphorylated (Ser/Thr/Tyr) PubMed

Cofactors

• lipoic acid (on Lys-43), can probably be removed by SrtN PubMed

Effectors of protein activity

• Inhibited by thiamine 2-thiothiazolone diphosphate and NADH PubMed
• Low sensibility to NADPH

Structure

• 1W88 (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus), 2PDE (peripheral subunit binding domain, Geobacillus stearothermophilus), 1LAC (lipoyl domain, Geobacillus stearothermophilus), 1B5S (catalytic domain (residues 184-425) , Geobacillus stearothermophilus)

Localization

• membrane associated PubMed
• cytoplasm (homogeneously distributed throughout the cell) PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: pdhA-pdhB-pdhC-pdhD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, due to presence of guanine at 1 position of the transcript PubMed, in stringent response

  Regulation

  • pdhA: expression activated by glucose (1.9-fold) PubMed

• Genes: pdhC-pdhD
  Description: PubMed

  Regulation

  • pdhA: expression activated by glucose (1.9-fold) PubMed

Biological materials

Mutant

• BKE14600 (pdhC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGTTCTCGACCTCCTAG, downstream forward: _UP4_TTAATTTTAATGGAGGCGTA
• BKK14600 (pdhC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGTTCTCGACCTCCTAG, downstream forward: _UP4_TTAATTTTAATGGAGGCGTA

Labs working on this gene/protein

• Arthur Aronson, Purdue University, West Lafayette, USA homepage

References

Reviews

Loading

Original publications

Loading